![]() ![]() La designacin 'USP NF 2014' en la cubierta de esta. Preparados por el Consejo de Expertos y sus Comits de Expertos Oficial desde el 7 de mayo de 20 74. FORMULARIO NACIONAL Autorizados por la Convencin de la Farmacopea de los Estados Unidos de Amrica. ![]() Locate the spots in the chromatogram by examination under short-wavelength UV light: any spot obtained from the solution under test, at an R F value corresponding to the principal spot from the Standard solution, is not greater in size or intensity than the principal spot obtained from the Standard solution, corresponding to not more than 0.001% of p-chloroacetanilide. FARMACOPEA DE LOS ESTADOS UNIDOS DE, AME RICA. Remove the plate from the developing chamber, mark the solvent front, and allow the solvent to evaporate. ![]() Develop the chromatogram in an unsaturated chamber, with a solvent system consisting of a mixture of solvent hexane and acetone (75:25), until the solvent front has moved three-fourths of the length of the plate. Similarly apply 40 µL of a Standard solution in ether containing 10 µg of p-chloroacetanilide per mL, and allow the spots to dry. Apply 200 µL of the supernatant, in 40-µL portions, to obtain a single spot not more than 10 mm in diameter to a suitable thin-layer chromatographic plate (see Chromatography 621 ) coated with a 0.25-mm layer of chromatographic silica gel mixture. Transfer 1.0 g to a glass-stoppered, 15-mL centrifuge tube, add 5.0 mL of ether, shake by mechanical means for 30 minutes, and centrifuge at 1000 rpm for 15 minutes or until a clean separation is obtained. ![]()
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